Creative Proteomics Released BrdU Cell Proliferation Assay to Be Behind Researchers
SHIRLEY, NY - Feb 21, 2020 - February 21, 2020, since its establishment in 2004, Creative Proteomics has served nearly 2 million researchers from different areas, including clinical, academia and industry. Recently, Creative Proteomics, as an excellent supplier proteomics, metabolomics, glycomics, and bioinformatics analysis fields, announced that the brdu (halogenated nucleotides such as the pyrimidine analog bromodeoxyuridine) cell proliferation assay service is available for customers to apply in their different research projects. With more than ten-year experience, Creative Proteomics allowed clients to enjoy custom services and these services are guaranteed to work well in different research projects.
BrdU cell proliferation assay experiment
The molecular weight of 5-brdu is 307.1. 100mg is divided into 30.7mg (0.1mmol) in three portions, dissolved in 1ml of triple distilled water, and stored in -20 ° C. When using it, dilute it 100 times, such as adding 10ul to 1ml solution. Pack as small as possible, such as 100ul, to avoid repeated freezing and thawing to reduce its activity.
1. Preparation of BrdU stock solution
BrdU 5mg (dissolved with 0.5ml 1N NaOH), and then add distilled water to 5ml to make a 1000ug / ml storage solution. BrdU will decompose when exposed to light. The storage solution should be stored in a brown bottle and sealed in a black paper to protect it from light. Generally, a final concentration of 10 μg / ml is used.
2. Before stopping cell culture, add BrdU (final concentration is 30 μg / L), incubate at 37 ° C for 40 min.
3. Discard the culture medium and wash the slides 3 times with PBS.
4. Methanol / acetic acid fixation for 10 min.
5. The endogenous oxidase was inactivated by 0.3% H2O2-methanol after air drying on fixed slides for 30 min.
6. 5% normal rabbit serum was blocked.
7. Denaturing the nucleic acid at 100 ° C for 5 min.
8. After cooling in an ice bath, wash with PBS, add 1 antibody to anti-mouse BrdU monoclonal antibody (working concentration 1:50), and add PBS or serum to the negative control.
9. According to the ABC method, hematoxylin or eosin was used for the staining, and the total number of cells and the number of BrdU positive cells in 10 high magnification fields were randomly counted under a microscope to calculate the labeling index (LI).
The cells were seeded at a cell number of 1.5 × 105 / ml in a 35 ml diameter dish (a cover glass was placed inside), cultured for 1 day, and synchronized with 0.4% FCS medium for 3 days, so that most cells were in the G0 phase.
scientists from Creative Proteomics contribute a lot to develop new services and high-quality products in order to meet different demands from customers. As a rapid, simple and high sensitive method to assess cell proliferation, BrdU cell proliferation assay developed by scientists in R&D of Creative Proteomics is very popular among our loyal clients. So it is our duty to guarantee its quality and function. said Dr. Armand, a senior scientist in Creative Proteomics.
About Creative Proteomics
As a leading supplier in proteomics bioinformatics, Creative Proteomics served researchers from academic and government organizations to industry. It is believed that Creative Proteomics has brighter future.